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M94A0738.TXT
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1994-10-21
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Document 0738
DOCN M94A0738
TI Role the immunodeficiency virus nef gene and the production in CNS
tropism.
DT 9412
AU Smith K; Deacon NJ; McPhee DA; Brew B; AIDS Molecular Biology Unit,
Macfarlane Burnet Centre, Vic.,; Australia.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:103 (poster no. 56).
Unique Identifier : AIDSLINE ASHM5/94348917
AB Particular strains of HIV may dominate the CNS of infected individuals
and it is possible that the HIV Nef protein plays a role in
differentiating CNS and peripheral blood isolates. To address this, 6
pairs of coincident isolates from the CSF and peripheral blood of
patients with AIDS or ARC were analysed for their ability to replicate
in primary blood mononuclear cells and primary macrophages. Peripheral
blood isolates appeared to have enhanced replication in all the cell
types examined but the replicative abilities of paired isolates were
very similar. CSF isolates were less likely to induce CPE and syncytia
in PBMC and macrophage cultures. Isolates were examined for their
ability to express the Nef protein by flow cytometry and immunoblotting
and the nef gene of isolates was PCR amplified and sequenced. Most
isolates appeared to express Nef and sequence analysis revealed little
difference in nef between CSF and blood isolates. No differences between
Nef of CSF and peripheral blood isolates were apparent by parameters
used in this study.
DE Acquired Immunodeficiency Syndrome/MICROBIOLOGY AIDS Dementia
Complex/*MICROBIOLOGY AIDS-Related Complex/MICROBIOLOGY
Brain/MICROBIOLOGY Cerebrospinal Fluid/MICROBIOLOGY Cytopathogenic
Effect, Viral/GENETICS Flow Cytometry Genes, nef/*GENETICS Human
HIV/*GENETICS Immunoblotting Polymerase Chain Reaction Sequence
Analysis Virus Replication/GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).